The new service, called Fleet Doctor, uses experts from top transport groups including motor research body TRL and former TNT and First Bus representatives, to provide fleets with an audit of operations.
Areas such as finance, performance, safety, economy, customer services and policies are scrutinised by the team of experts who then go on to produce a report of findings.
Fleet Doctor is the brainchild of several members of transport research group TRL and is headed by Alan Parker, formerly engineering director at TNT and First Bus.
Saul Jeavons, one of the experts, is manager of TRL's Risk Management Group and responsible for TRL's component of the Department for Transport's Fuel Economy Advisers' Scheme.
He said: 'We believe the unique thing about Fleet Doctor is the team behind it, a mix of researchers and industry experts with a broad range of expertise and experience in all factors affecting fleet operations from corporate liability issues through maintenance, fuel economy, corporate finance and component life.
'The team offers the operator a completely impartial and independent voice, giving objective advice on the health of their operation. This mix of researchers and practitioners is unique in the industry.'
A typical audit from Fleet Doctor usually takes two days to complete and will cost about £2,000 to £4,000. Smaller fleets of about five vehicles will be looking to pay £1,500.
Fleet Doctor claims fleets can save money, improve efficiency and protect against criminal prosecution by completing the audit.
However, the actual monetary savings depend on individual fleet circumstances.
Jeavons said: 'The amount fleets can save depends on how well or badly the fleet is being run in the first instance. What type of operation they are running in terms of nature of business, type of vehicles, maintenance and financing arrangements.'