The Scottish Ambulance Service will receive £34.3 million over the next four years to replace more than 570 vehicles.
This will be made up of 326 new accident and emergency vehicles – comprising of traditional ambulances and paramedic response units - 98 patient transport vehicles and 151 support vehicles.
This includes 65 paramedic response units – these are cars or motorbikes that are used to get a paramedic to a patient as quickly as possible, often backed up by a traditional ambulance.
Health secretary Nicola Sturgeon said: “Our hardworking Ambulance staff do a fantastic job and are out 365 days a year saving lives.
“They cannot do this without modern, up to date mix of vehicles that allow them to provide the very best patient care to the people of Scotland.
Pauline Howie, chief executive, Scottish Ambulance Service, said: "Our crews deliver the highest standard of patient care in a wide range of conditions and environments so it is essential that they operate with the most appropriate equipment.
“The funding for our vehicle fleet ensures that care continues to be delivered in ambulances that are fit for purpose and specifically designed to meet modern healthcare standards."