Called 'Walk in to Work Out', the scheme is aimed at companies wanting to give employees information about walking or cycling to work.
The free information pack includes a guide for co-ordinators, booklets for staff and promotional posters. It contains details on goal setting, journey planning and safety.
According to the DTLR, a recent survey suggested that many motorists who drive short distances to work would consider walking or cycling for their health, and of those who already have switched, 34% did so for their health, 8% for environmental concern and 2% to help reduce congestion.
The pack has been launched on the back of a successful pilot in Scotland that doubled the amount of employees choosing to leave their cars at home.
A spokesman for the DTLR said: 'We would welcome fleet managers who want to get involved with the scheme. It's a great idea for improving health, and helping the environment.'