The two companies have signed a contract that encompasses 170 vehicles, including 100 on a sale and leaseback basis.
Alan Miles, RNIB's administration and data protection manager, who is responsible for the fleet, said: 'The certainty of fixed costs through contract hire was an attractive option for us, as well as being able to include sale and leaseback for about 100 vehicles from our previous outright purchase arrangement.
'We are also delighted with the level of choice and range of services offered to our drivers, which really helps reduce the time spent on internal administration.'
RNIB's contract includes maintenance, direct driver contact, accident management and the ServiceTrak maintenance and repair facility.
Lloyds TSB autolease regional business development manager Rod Allen said: 'We worked closely with the RNIB over a number of years to create exactly the right solution to meet its needs. Its fleet deal provides more certainty by eliminating fluctuating costs and reduces the administration burdens which is exceptionally helpful when running a charitable organisation.'